7CC3
Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: co-FG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.048, 99.221, 193.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.062 - 1.720 |
| Rwork | 0.196 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7caq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.052 | 1.840 |
| Number of reflections | 202517 | 9927 |
| <I/σ(I)> | 42.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 22.3 | |
| CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.075 M succinic acid, pH7.0, 11% PEG 3350, and 25% glycerol |
