7CC3
Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: co-FG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.048, 99.221, 193.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.062 - 1.720 |
Rwork | 0.196 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7caq |
RMSD bond length | 0.004 |
RMSD bond angle | 1.252 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.052 | 1.840 |
Number of reflections | 202517 | 9927 |
<I/σ(I)> | 42.6 | |
Completeness [%] | 100.0 | |
Redundancy | 22.3 | |
CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.075 M succinic acid, pH7.0, 11% PEG 3350, and 25% glycerol |