7CBE
Crystal structure of Homoserine O-succinyltransferase from Escherichia coli K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.348, 81.027, 98.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.750 - 1.700 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h2w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.075 | 0.056 | 0.282 |
| Rmeas | 0.081 | 0.061 | 0.324 |
| Rpim | 0.031 | 0.023 | 0.154 |
| Total number of observations | 342170 | ||
| Number of reflections | 65291 | 3494 | 3176 |
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 97.0 | 96.3 | 95.8 |
| Redundancy | 5.2 | 6.7 | 3.7 |
| CC(1/2) | 0.996 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 283 | 25% Polyethylene glycol 3350, 0.1M Bis-tris pH 5.5, 0.2M Ammonium sulfate |
