7CB8
Structure of a FIC-domain protein from Mycobacterium marinum in complex with CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97990 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.066, 107.900, 110.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.488 - 2.600 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zcn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1-2575-000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.210 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.202 | 0.871 |
Rmeas | 0.230 | 0.992 |
Rpim | 0.108 | 0.460 |
Number of reflections | 27934 | 3389 |
<I/σ(I)> | 6.5 | 1.6 |
Completeness [%] | 97.8 | 99 |
Redundancy | 4.1 | 4.2 |
CC(1/2) | 0.980 | 0.605 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 293 | 0.2M Ammonium sulfate, 0.1M Sodium acetate trihydrate, and 25% (w/v) PEG 4,000 |