7C99
Human DMC1 post-synaptic complexes with mismatched dsDNA
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.36 |
| Number of Particle | 140823 |
| Software | Coot,Rosetta,RELION,EPU,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
