7C95

Crystal structure of the anti-human podoplanin antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	53.720, 65.850, 117.750
Unit cell angles	90.00, 97.27, 90.00

Refinement procedure

Resolution	46.320 - 2.130
R-factor	0.2057
Rwork	0.204
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3l
RMSD bond length	0.003
RMSD bond angle	0.607
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.320	46.320	2.260
High resolution limit [Å]	2.130	6.340	2.130
Rmerge	0.097	0.039	0.639
Number of reflections	44820	1825	6701
<I/σ(I)>	17.3	52.04	2.15
Completeness [%]	97.7	99.7	90.4
Redundancy	11.668
CC(1/2)	0.999	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M Tris-HCl, 0.2M magnesium Acetate, 25% v/v PEG 3350