7C90
Crystal structure of Cytochrome CL from the marine methylotrophic bacterium Methylophaga aminisulfidivorans MPT (Ma-CytcL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97941 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.760, 76.060, 66.440 |
Unit cell angles | 90.00, 106.85, 90.00 |
Refinement procedure
Resolution | 30.580 - 2.130 |
R-factor | 0.1509 |
Rwork | 0.148 |
R-free | 0.19980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d0w |
RMSD bond length | 0.010 |
RMSD bond angle | 2.113 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.170 |
High resolution limit [Å] | 2.130 | 5.780 | 2.130 |
Rmerge | 0.103 | 0.060 | 0.630 |
Rmeas | 0.111 | 0.065 | 0.678 |
Rpim | 0.042 | 0.026 | 0.248 |
Total number of observations | 237733 | ||
Number of reflections | 32914 | 1676 | 1595 |
<I/σ(I)> | 11.7 | ||
Completeness [%] | 99.9 | 97.8 | 100 |
Redundancy | 7.2 | 6.1 | 7.4 |
CC(1/2) | 0.996 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1600 mM sodium/potassium phosphate monobasic, 100 mM HEPES/sodium hydroxide pH 7.5 |