7C7O
Crystal structure of E.coli DNA gyrase B in complex with 6-fluoro-8-(methylamino)-2-oxo-1,2-dihydroquinoline derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.251, 67.585, 67.913 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.610 - 1.800 |
| R-factor | 0.17309 |
| Rwork | 0.170 |
| R-free | 0.22733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aj6 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.610 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.286 |
| Number of reflections | 18009 | 1730 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M MES pH6.0, 0.2M Ammonium acetate, 24% PEG3350 |
