7C5F

Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase1 from Escherichia coli at 1.88 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	89.651, 89.651, 341.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.810 - 1.880
Rwork	0.180
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k73
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.880	1.880
Rmerge	0.200	1.800
Rpim		0.637
Number of reflections	114194	114194
<I/σ(I)>	2	13.5
Completeness [%]	99.9	100
Redundancy	8.9	9.8
CC(1/2)		0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100mM sodium acetate pH4.6, 30%(w/v) PEG 400, 200mM Ca(OAC)2