7C4C

The crystal structure of Trypanosoma brucei RNase D : GMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	57.169, 75.001, 101.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.265
R-factor	0.1946
Rwork	0.192
R-free	0.23890
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.150	30.000	2.330
High resolution limit [Å]	2.250	4.840	2.250
Rmerge	0.089	0.050	0.405
Rmeas	0.095	0.053	0.466
Rpim	0.032	0.015	0.221
Number of reflections	20606	2241	1905
<I/σ(I)>	9.2
Completeness [%]	98.3	99.5	92.5
Redundancy	7.3	10.8	3.6
CC(1/2)		0.998	0.174

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 30% PEG 3350