7C45

The crystal structure of Trypanosoma brucei RNase D complex with RNA U12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	44.449, 65.843, 99.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.840 - 1.769
R-factor	0.194
Rwork	0.193
R-free	0.22120
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.769	3.810	1.770
Rmerge	0.079	0.036	0.473
Rmeas	0.090	0.041	0.546
Rpim	0.043	0.020	0.265
Number of reflections	28011	2946	2580
<I/σ(I)>	5.5
Completeness [%]	95.2	93.4	90.2
Redundancy	4.1	4	3.9
CC(1/2)		0.995	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Sodium acetate trihydrate, 20% PEG 3350