7C43

The crystal structure of Trypanosoma brucei RNase D : AMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	49.730, 77.730, 99.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.210 - 2.300
R-factor	0.2021
Rwork	0.200
R-free	0.24240
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.210	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.071	0.053	0.279
Rmeas	0.074	0.056	0.307
Rpim	0.023	0.016	0.122
Number of reflections	17294	1899	1594
<I/σ(I)>	8.9
Completeness [%]	97.4	99	91.6
Redundancy	8.4	11.2	4.8
CC(1/2)		0.998	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 30% PEG 3350