7C40
MgGDP bound KRAS G12V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 |
Unit cell lengths | 82.547, 82.547, 40.804 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.017 - 2.516 |
R-factor | 0.177 |
Rwork | 0.174 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tq9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.077 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.169 |
High resolution limit [Å] | 2.500 | 2.516 |
Rmerge | 0.128 | 0.515 |
Number of reflections | 5543 | 5527 |
<I/σ(I)> | 27.2 | |
Completeness [%] | 99.9 | |
Redundancy | 21.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Polyethylene glycol 3,350, 0.2M potassium nitrate (pH 6.8) |