7C3K

Crystal Structure of mIRGB10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9779
Spacegroup name	P 1 21 1
Unit cell lengths	62.510, 63.190, 117.920
Unit cell angles	90.00, 98.70, 90.00

Refinement procedure

Resolution	29.140 - 2.600
Rwork	0.213
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tpa
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.140	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.076	0.430
Rmeas	0.082	0.463
Rpim	0.031	0.170
Number of reflections	28239	2784
<I/σ(I)>	17.57	3.95
Completeness [%]	99.8	99.82
Redundancy	7	7.3
CC(1/2)	0.999	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 8,000, HEPES