7C3F
Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin m2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.262, 137.209, 192.457 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 43.572 - 2.399 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2puk |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.572 | 2.485 |
| High resolution limit [Å] | 2.399 | 2.399 |
| Rmerge | 0.123 | 1.406 |
| Rmeas | 0.144 | 1.635 |
| Rpim | 0.074 | 0.830 |
| Number of reflections | 175388 | 17050 |
| <I/σ(I)> | 8.82 | 1.19 |
| Completeness [%] | 99.1 | 96.86 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.995 | 0.493 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277.15 | 100 mM NaCitrate pH 6.2 18% PEG 3,350 |
