7C06
Crystal structure of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.823, 255.101, 94.112 |
Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
Resolution | 38.410 - 3.020 |
R-factor | 0.2162 |
Rwork | 0.215 |
R-free | 0.24870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yh8 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.511 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 3.080 |
High resolution limit [Å] | 3.020 | 3.020 |
Rmerge | 0.113 | 1.040 |
Number of reflections | 84760 | 4562 |
<I/σ(I)> | 17 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.7 |
CC(1/2) | 0.997 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M NaCitrate (pH 6.0), 10% PEG 35000 |