7BYJ

Crystal structure of the FERM domain of FRMPD4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97890
Spacegroup name	P 43 21 2
Unit cell lengths	49.149, 49.149, 350.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.300 - 2.490
R-factor	0.2284
Rwork	0.224
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e2q
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.300	45.300	2.640
High resolution limit [Å]	2.490	7.390	2.490
Rmerge	0.087	0.048	1.014
Rmeas	0.089	0.049	1.034
Total number of observations	382398
Number of reflections	16231	747	2514
<I/σ(I)>	23.79	57.67	2.7
Completeness [%]	100.0	99.6	99.9
Redundancy	23.56	19.552	25.812
CC(1/2)	0.999	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Lithium citrate tribasic tetrahydrate, Polyethylene glycol 3350