7BY6
Plasmodium vivax cytoplasmic Phenylalanyl-tRNA synthetase in complex with BRD1389
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9688 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.523, 74.071, 121.682 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 121.782 - 2.997 |
R-factor | 0.2176 |
Rwork | 0.214 |
R-free | 0.28820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l4g |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 136.500 | 3.050 |
High resolution limit [Å] | 2.997 | 3.000 |
Rmeas | 0.145 | 1.288 |
Number of reflections | 25453 | 1186 |
<I/σ(I)> | 8.5 | 1.6 |
Completeness [%] | 99.6 | 94.6 |
Redundancy | 6.3 | 6.3 |
CC(1/2) | 0.921 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, 3% w/v poly-gama-glutamic acid (Na+ form, low molecular weight), 3% w/v PEG20000, 0.1 M ammonium sulphate, 0.3 M sodium formate |