7BXO
Crystal structure of the toxin-antitoxin with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.510, 100.680, 132.510 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 36.140 - 2.770 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yep |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.680 | 2.860 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 36307 | 2668 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M Tris-HCl , 0.2 M potassium sodium tartrate, and 12-20% (v/v) PEG 3350 |
