7BXG

MavC-UBE2N-Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 65
Unit cell lengths	150.527, 150.527, 58.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.111 - 2.705
R-factor	0.1977
Rwork	0.193
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc 5zq3 5eya
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.112	0.541
Number of reflections	36277	1038
<I/σ(I)>	29
Completeness [%]	100.0
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium malonate, pH 7.0, 12% PEG3350