7BVY
Crystal structure of MreB 5 of Spiroplasma citri bound to AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-01 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 51.121, 54.083, 138.800 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 49.838 - 2.500 |
R-factor | 0.1931 |
Rwork | 0.190 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bvz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.454 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.838 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.200 | 0.890 |
Rpim | 0.120 | 0.540 |
Number of reflections | 13181 | 1461 |
<I/σ(I)> | 7 | 2.2 |
Completeness [%] | 98.9 | 98.3 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 291 | 0.15M Na-K phosphate, 16% PEG 3350, pH 7.8, 2mM AMPPNP, 2mM MgCl2 |