7BV4
Crystal structure of STX17 LIR region in complex with GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 41 |
| Unit cell lengths | 42.943, 42.943, 245.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.540 - 2.000 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yir |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.540 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.600 |
| Number of reflections | 29800 | 2976 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.12M Alcohols, 50% (v/v) Precipitant Mix 4, Buffer System 3 at pH 8.5 in Morpheus screen kit from Molecular Dimension |
