7BSR
Mandelate oxidase with the 2-Hydroxy-3-oxosuccinic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 138.440, 138.440, 109.144 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.228 - 1.894 |
R-factor | 0.2221 |
Rwork | 0.220 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sgz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.890 | 1.894 |
Rmerge | 0.039 | 0.745 |
Number of reflections | 41750 | 4154 |
<I/σ(I)> | 36.4 | 2.6 |
Completeness [%] | 98.9 | |
Redundancy | 9.7 | |
CC(1/2) | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 35% Tascimate, 0.1M Bis-Tris propane pH 7.0 |