7BRU

Crystal structure of human RTN3 LIR fused to human GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	137.418, 137.418, 67.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.131 - 2.149
R-factor	0.2074
Rwork	0.206
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.008
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.131	2.226
High resolution limit [Å]	2.149	2.149
Rmerge	0.072	0.838
Rmeas	0.075	0.871
Rpim	0.021	0.236
Number of reflections	35698	3473
<I/σ(I)>	20.14	2.43
Completeness [%]	99.9	99.28
Redundancy	13.2	13.4
CC(1/2)	0.999	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.8 M potassium phosphate monobasic, 0.8 M sodium phosphate monobasic, 0.1 M HEPES pH 7.5