7BOC
Crystal structure of the PRMT5 TIM barrel domain in complex with RioK1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-17 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.999859 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 96.630, 96.630, 112.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.310 - 2.550 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4x60 |
Data reduction software | XDS (VERSION Jan 31, 2020 BUILT=20200417) |
Data scaling software | XSCALE (VERSION Jan 31, 2020 BUILT=20200417) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18-3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.310 | 48.310 | 2.620 |
High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
Rmerge | 0.191 | 0.066 | 4.289 |
Rmeas | 0.196 | 0.068 | 4.395 |
Total number of observations | 409271 | ||
Number of reflections | 20297 | 266 | 1496 |
<I/σ(I)> | 12.05 | 34.48 | 1.27 |
Completeness [%] | 99.9 | 98.9 | 99.9 |
Redundancy | 20.164 | 16.425 | 21.144 |
CC(1/2) | 0.999 | 0.998 | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1M NaAcetate, 30% w/v PEG400, 0.2M CaAcetate |