7BM5
Crystal structure of Fab1, the Fab fragment of the anti-BamA monoclonal antibody MAB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.014, 130.144, 138.921 |
| Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
| Resolution | 88.423 - 2.950 |
| R-factor | 0.2585 |
| Rwork | 0.257 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zan |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.189 | 93.189 | 3.426 |
| High resolution limit [Å] | 2.950 | 10.513 | 2.955 |
| Rmerge | 0.422 | 0.084 | 1.058 |
| Rmeas | 0.457 | 0.091 | 1.157 |
| Rpim | 0.173 | 0.035 | 0.459 |
| Total number of observations | 10236 | 9099 | |
| Number of reflections | 30473 | 1522 | 1525 |
| <I/σ(I)> | 3.6 | 8.8 | 1.6 |
| Completeness [%] | 92.1 | 99.6 | 68.8 |
| Redundancy | 6.8 | 6.7 | 6 |
| CC(1/2) | 0.978 | 0.996 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.16 M lithium chloride, 22% (w/v) PEG6000, 0.1 M MES pH 6.0 |
