7BLN
VPS35/VPS29 arch of metazoan membrane-assembled retromer:SNX3 complex modelled with human proteins
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SUBTOMOGRAM AVERAGING |
Resolution (Å) | 8.9 |
Software | PHENIX,UCSF Chimera |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |