7BL1
human complex II-BATS bound to membrane-attached Rab5a-GTP
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 9.8 |
| Software | MATLAB,RELION,REFMAC,ISOLDE,SerialEM,NOVACTF,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
