7BEA

Structure of human Programmed cell death 1 ligand 1 (PD-L1) with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 2 21 21
Unit cell lengths	32.617, 54.616, 140.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 2.450
Rwork	0.274
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o45
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.138
Number of reflections	9843	956
<I/σ(I)>	10.9
Completeness [%]	99.8	98.9
Redundancy	7.1
CC(1/2)	0.997	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	1.2 M Sodium citrate tribasic dihydrate 0.01 M Sodium borate, pH 8.5