7BDX
Armadillo domain of HSF2BP in complex with BRCA2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.887, 135.830, 75.794 |
Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
Resolution | 49.580 - 2.600 |
R-factor | 0.1997 |
Rwork | 0.198 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | H3 domain from Robetta |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.600 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.158 | |
Rpim | 0.062 | |
Number of reflections | 30850 | 930 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 83.7 | |
Redundancy | 7.4 | |
CC(1/2) | 0.998 | 0.355 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG 3350 : 16% (w/v) Mes ph 6.0 100 mM MgCl2 100 mM |