7BDK

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	99.532, 118.620, 187.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.090 - 2.520
R-factor	0.2297
Rwork	0.228
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.500	2.500
Rmeas	0.263
Number of reflections	74606	11542
<I/σ(I)>	7.4
Completeness [%]	98.6	95.6
Redundancy	6.7	6.7
CC(1/2)	0.993	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350