7BCS
ASCT2 in the presence of the inhibitor Lc-BPE (position "down") in the outward-open conformation.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.43 |
Number of Particle | 3666842 |
Software | cryoSPARC,RELION,PHENIX,EPU,CTFFIND,Coot,UCSF Chimera |
Symmetry | C3 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |