7B92

Structure of a minimal SF3B core in complex with sudemycin D6 (form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	107.429, 107.429, 361.474
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.520 - 3.000
R-factor	0.2251
Rwork	0.223
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ife 6en4
RMSD bond length	0.003
RMSD bond angle	0.537
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.060	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.181	4.108
Rmeas	0.185	4.208
Rpim	0.041	0.907
Number of reflections	49623	4468
<I/σ(I)>	14.7	0.8
Completeness [%]	100.0	100
Redundancy	20.8	21.3
CC(1/2)	0.999	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M HEPES pH=7.42, 0.2 M Magnesium chloride, 27.75% (v/v) PEG-400