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7B5G

Crystal structure of E.coli LexA in complex with nanobody NbSOS3(Nb14527)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04-1
Synchrotron siteDiamond
BeamlineI04-1
Temperature [K]100
Detector technologyPIXEL
Collection date2019-05-10
DetectorDECTRIS EIGER2 XE 16M
Wavelength(s)0.979499
Spacegroup nameP 1
Unit cell lengths49.067, 57.081, 122.506
Unit cell angles84.53, 83.21, 68.43
Refinement procedure
Resolution49.610 - 2.400
Rwork0.206
R-free0.25200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1jhf
Data reduction softwarexia2
Data scaling softwarexia2
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]53.0602.490
High resolution limit [Å]2.4002.400
Rmerge0.0840.730
Number of reflections470614685
<I/σ(I)>5.91
Completeness [%]98.2
Redundancy2.4
CC(1/2)0.923
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.82930.15 M sodium chloride, 0.05 M HEPES, 24 % w/v PEG3350

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