7B5G
Crystal structure of E.coli LexA in complex with nanobody NbSOS3(Nb14527)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-10 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.979499 |
Spacegroup name | P 1 |
Unit cell lengths | 49.067, 57.081, 122.506 |
Unit cell angles | 84.53, 83.21, 68.43 |
Refinement procedure
Resolution | 49.610 - 2.400 |
Rwork | 0.206 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jhf |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.060 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.084 | 0.730 |
Number of reflections | 47061 | 4685 |
<I/σ(I)> | 5.9 | 1 |
Completeness [%] | 98.2 | |
Redundancy | 2.4 | |
CC(1/2) | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.15 M sodium chloride, 0.05 M HEPES, 24 % w/v PEG3350 |