7B57
Crystal structure of CaMKII-actinin complex bound to ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9119 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.918, 71.849, 91.602 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.530 - 1.950 |
R-factor | 0.2059 |
Rwork | 0.203 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vz6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.885 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.530 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.059 | 0.960 |
Number of reflections | 32429 | 32429 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 100.0 | |
Redundancy | 6.3 | |
CC(1/2) | 0.967 | 0.487 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M MES pH 6.0, 20% w/v PEG4000, 0.2 M Lithium Sulfate |