7B44
Crystal structure of c-MET bound by compound S1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.120, 42.550, 85.790 |
| Unit cell angles | 90.00, 121.30, 90.00 |
Refinement procedure
| Resolution | 23.010 - 1.760 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | internal |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmeas | 0.073 | 1.049 |
| Rpim | 0.040 | 0.666 |
| Total number of observations | 99230 | |
| Number of reflections | 32999 | 1425 |
| <I/σ(I)> | 10.8 | 1 |
| Completeness [%] | 97.0 | 82.9 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25 % PEG3350, 0.2 M (NH4)2SO4, 0.1 M Na-HEPES pH 7.5 |
