7B04
Structure of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.7345 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 177.626, 206.467, 527.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 192.250 - 2.970 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | STARANISO (v3.000) |
| Phasing software | PHENIX (v1.14rc1) |
| Refinement software | PHENIX (v1.14rc1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 206.470 | 3.070 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.428 | 2.480 |
| Rmeas | 0.444 | 2.608 |
| Rpim | 0.120 | 0.786 |
| Number of reflections | 377277 | 18807 |
| <I/σ(I)> | 9.6 | 1.4 |
| Completeness [%] | 96.7 | 62.1 |
| Redundancy | 26.3 | 20.6 |
| CC(1/2) | 0.990 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 13% PEG 4000 50 mM TrisCl pH 8.5 1.8 mM n-Decyl-beta-D-maltoside |
