7AZX
Crystal structure of the MIZ1-BTB-domain in complex with a HUWE1-derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-20 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.110, 69.110, 97.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.560 - 2.250 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m52 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 2.400 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.066 | 0.684 |
| Number of reflections | 9023 | 450 |
| <I/σ(I)> | 13.8 | 1.5 |
| Completeness [%] | 68.0 | 18.8 |
| Redundancy | 34.2 | 27 |
| CC(1/2) | 0.999 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 4000, sodium acetate |
