7AZO
70S thermus thermophilus ribosome with bound antibiotic lead SEQ-977
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.770, 446.870, 616.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 253.870 - 3.300 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v6f |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 0.420 | 3.130 |
| Number of reflections | 860556 | 73202 |
| <I/σ(I)> | 9.72 | |
| Completeness [%] | 100.0 | |
| Redundancy | 30 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG2000MMES, KSCN |
