7AZF

DNA polymerase sliding clamp from Escherichia coli with peptide 8 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	70.418, 82.034, 82.392
Unit cell angles	116.87, 100.26, 95.47

Refinement procedure

Resolution	37.750 - 1.930
R-factor	0.206
Rwork	0.204
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.700	1.950
High resolution limit [Å]	1.920	1.920
Rpim	0.079	0.727
Number of reflections	84971	99
<I/σ(I)>	8.8	1.1
Completeness [%]	87.8
Redundancy	6.8
CC(1/2)	0.990	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M DL Malic acid pH 7.0, PEG 3350 20% (w/v)