7AZ7
DNA polymerase sliding clamp from Escherichia coli with peptide 37 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.180, 67.620, 81.520 |
Unit cell angles | 90.00, 128.09, 90.00 |
Refinement procedure
Resolution | 53.990 - 1.650 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.990 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rpim | 0.027 | 0.680 |
Number of reflections | 39718 | 61 |
<I/σ(I)> | 14.5 | 1.1 |
Completeness [%] | 86.7 | |
Redundancy | 13 | |
CC(1/2) | 0.990 | 0.180 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2 M Ammonium formate, pH 6.6, PEG 3350 20% (w/v) |