7AYZ

Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with activator TH10785

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9780
Spacegroup name	P 21 21 21
Unit cell lengths	80.833, 81.802, 170.643
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.873 - 2.600
Rwork	0.237
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g3y
RMSD bond length	0.003
RMSD bond angle	1.227
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.300	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.082	0.986
Rmeas	0.088	1.070
Rpim	0.034	0.412
Number of reflections	35633	4269
<I/σ(I)>	18.3	2.2
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.06 M Divalents, 0.1 M Buffer System 3 pH 8.5, 30 % v/v Precipitant Mix 2 (Morpheus screen, Molecular Dimensions)