7AVE
Perdeuterated refolded hen egg-white lysozyme at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-15 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.670, 30.970, 33.740 |
| Unit cell angles | 89.44, 72.82, 67.50 |
Refinement procedure
| Resolution | 32.010 - 0.980 |
| R-factor | 0.1052 |
| Rwork | 0.105 |
| R-free | 0.11450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yeo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.15.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.010 | 1.015 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.042 | 0.273 |
| Rmeas | 0.053 | 0.306 |
| Rpim | 0.047 | 0.136 |
| Number of reflections | 52966 | 5018 |
| <I/σ(I)> | 18.09 | 4.21 |
| Completeness [%] | 97.4 | 92.67 |
| Redundancy | 5.3 | 4.9 |
| CC(1/2) | 0.999 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.5 | 291 | 0.15 M sodium nitrate, 0.05 M sodium acetate pH 4.5 |
