7AU7

Crystal structure of Nod Factor Perception ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	77.410, 98.160, 71.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.080 - 2.547
R-factor	0.2112
Rwork	0.205
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ebz
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.080	2.620
High resolution limit [Å]	2.547	2.547
Rmerge	0.155
Rmeas	0.168
Number of reflections	9247	136
<I/σ(I)>	10.7	1.95
Completeness [%]	79.6	20
Redundancy	6.74
CC(1/2)	0.996	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292.15	0.2 M Na-acetate * 3H2O, 0.1 M Na-cacodylate pH 6.5, 30% (w/v) PEG-8000,