7AOV
Crystal Structure of a TRPM2 Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.760, 188.128, 47.773 |
Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
Resolution | 46.612 - 2.000 |
R-factor | 0.190409700336 |
Rwork | 0.188 |
R-free | 0.23118 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.927 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.620 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 53708 | 5374 |
<I/σ(I)> | 10.28 | 1.46 |
Completeness [%] | 97.2 | 96.91 |
Redundancy | 13.9 | |
CC(1/2) | 0.996 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | Tris, BICINE, Diethylene glycol, Triethylene glycol, Tetraethylene glycol, Pentaethylene glycol, glycerol, PEG4000, Jeffamine M-600 |