7AMA
IL-17A in complex with small molecule modulators
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.360, 85.200, 118.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.840 - 2.480 |
| R-factor | 0.21262 |
| Rwork | 0.210 |
| R-free | 0.25469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.988 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.840 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 12251 | 579 |
| <I/σ(I)> | 24 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.79 | |
| CC(1/2) | 1.000 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 10 000, isopropanol, sodium citrate |
