7ALX
Sav-SOD: Chimeric Streptavidin-cSOD as Host for Artificial Metalloenzymes
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 102.525, 102.525, 59.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.893 - 1.800 |
Rwork | 0.179 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4irw |
RMSD bond length | 0.015 |
RMSD bond angle | 1.973 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.893 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 29784 | 1719 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.4 | |
CC(1/2) | 0.999 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 %w/v PEG 3350 (Precipitant) 0.2 M Na K Phos (Salt) 0.1 M BIS-TRIS prop 7.5 pH (Buffer) |