7ALB
human GCH-GFRP stimulatory complex 7-deaza-GTP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 160.656, 116.137, 177.316 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 160.060 - 1.979 |
| R-factor | 0.2064 |
| Rwork | 0.206 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (18-SEP-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 160.060 | 2.139 |
| High resolution limit [Å] | 1.979 | 1.979 |
| Number of reflections | 2125175 | 346319 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 93.4 | 68.9 |
| Redundancy | 5.2 | |
| CC(1/2) | 0.998 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 25% MPD 0.2 ; NH4 Acet; 0.1 M Bis-Tris pH 5.5 |
