7AL4
Ancestral Flavin-containing monooxygenase (FMO) 1 (mammalian)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.919, 92.453, 156.688 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 49.100 - 3.000 |
| R-factor | 0.22913 |
| Rwork | 0.228 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6se3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 3.041 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.245 | 7.993 |
| Number of reflections | 39983 | 567 |
| <I/σ(I)> | 5.5 | 1.1 |
| Completeness [%] | 60.2 | 14.1 |
| Redundancy | 6.6 | 5 |
| CC(1/2) | 0.994 | 0.028 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 100 mM HEPES (pH 7.5), PEG 4000 (10% v/v) and glycerol (20% v/v) |
