Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 81.870, 81.870, 355.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.382 - 3.100 |
| Rwork | 0.249 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gzl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.087 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.382 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 13493 | 2398 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 97.9 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.990 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 279 | 8% v/v PEG4,000 0.1M Tris pH 8.5 0.2M Sodium acetate |
