7AHU

Anti-FX Fab of mim8 in complex with human FXa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	63.130, 105.250, 145.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.590 - 2.600
R-factor	0.2139
Rwork	0.203
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i1k 1g2l
RMSD bond length	0.011
RMSD bond angle	1.880
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.590	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.121	1.024
Rmeas	0.135	1.138
Rpim	0.059	0.493
Number of reflections	58767	30703
<I/σ(I)>	12.31
Completeness [%]	99.9	99.97
Redundancy	5.2	5.2
CC(1/2)	0.996	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, 18 % (w/v) PEG 8000